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Your powerful scientific imaging software

arivis Vision4D is your modular software for working with multi-channel 2D, 3D and 4D images of almost unlimited size, highly scalable and independent of local system resources. Many modern microscope systems such as high-speed confocal, light sheet / SPIM, super-resolution, electron microscopy or X-ray instruments can produce huge amounts of imaging data. arivis Vision4D gives you the power to handle your datasets without constraints and get your results in next to no time.

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  • Import of most imaging formats from microscopes and imaging instruments
  • A user-friendly interface that is easy to learn and use for any life scientist
  • Intuitive stitching and alignment to create large multi-dimensional image stacks
  • Immediate visualization, annotation and analysis on workstations and notebooks in 2D, 3D and 4D, regardless of image size
  • Magic Wand for easy semi-automatic creation of objects in 2D and 3D mode
  • Powerful Analysis Pipeline for 3D /4D image analysis (cell segmentation, tracking, annotation, quantitative measurement and statistics, etc)
  • Easy creation and export of 3D / 4D high resolution movies for publication
  • Seamless integration of custom workflows via Matlab API and Python scripting

The power of Vision4D

3D animated thoracic and abdominal ganglia of cockroach, created with arivis Vision4D.

Courtesy of M. Paoli, Galizia Lab, University of Konstanz, Germany.


Image acquisition with ZEISS Airyscan confocal microscopy. Brain, thoracic and abdominal ganglia of the cockroach are joined together by bilateral connective bundles of ascending and descending interneurons forming the ventral nerve cord. In this preparation, left and right connectives were individually labelled (Alexa 488: green, Alexa 647: magenta) posteriorly to the suboesophageal ganglion to observe the extension of their innervation within the different neurophils, and throughout the ipsi- and contralateral parts of the brain (DNA labelled with DAPI: cyan). Imaging was performed using Tiling and Stitching to capture the complete volume (3×2.3× 0.26 mm). 3D animation of the complete dataset was done with arivis Vision 4D, ideal for rendering and analyzing large datasets. The 4D viewer can be configured to adjust the appearance of individual channels independently to highlight specific features.

Optional Modules

Tracking & Lineage Module

Tracking cells or sub-cellular particles in microscopy data can be challenging. The arivis
Vision4D Tracking & Lineage Module allows the tracking of objects throughout time series in both 2D or 3D image sets of any size. Vision4D offers an easy to use approach with full control and interactivity at all steps. Several automatic algorithms allow fast and reproducible tracking which can be readily applied to other data sets. When automatic algorithms meet their limitations, use Virtual Reality with VisionVR to proof read and correct your automatically created results much more efficiently than ever before.

Batch Analysis Module

The arivis Vision4D Batch Analysis Module can be used for the unattended execution of a pre-configured analysis pipeline on a series of images or regions of interest. Analysis pipelines created in the arivis Analysis Module can easily be applied to multiple datasets. For routine workflows and inexperienced users, predefined Analysis Pipelines can be used and adapted easily.

Exchange Objects from Open Source and ZEISS ZEN

The arivis Vision4D Exchange Objects Module allows to easily interface with your specific workflows whenever you are using segmentation functions in open source, such as FiJI, ImageJ or Ilastik. Import these results with a single step and visualize or further analyze your 3D objects to measure e.g. volumes or count vesicles within cells or ROIs. Create high quality renderings for publications from segmentation results from other programs. The 2D/3D split views allow interactive exploration of the results in 3D with feature-based color coding or interactive gating in scatter plots or time line graphs.

Volume Fusion

This tool allows to fuse two image sets at any arbitrary angle in 3D. You can rotate and translate one image set against another image set and merge them to create a fused volume for further processing, rendering or analysis. Settings for the rotation, translation and scaling of the volumes can easily be configured using landmark registration or by rotating a surface extracted from the moving volume directly in the 4D viewer. The ability to merge volumes into new channels or to blend them into the existing volume data adds a high level of flexibility for further processing workflows.

arivis Storyboard

With arivis Storyboard, you easily create high-resolution movies to produce impressive sequences in 3D or 3D time series. Views, render modes and specific time points as well as the use of clipping planes or varying opacities of individual channels can be stored as key frames. arivis Vision4D automatically interpolates between these key frames to produce a seamless animation. These animations can be previewed and edited prior to producing high resolution video renderings.


Natural exploration of neurons in the Drosophila brain

Image Courtesy of Chris Zugates, Interdisciplinary Project Team "Fly Light", Janelia Research Campus

Vision4D in Detail

Import arivis Vision4D image support is vendor-independent and enables users to work with multimodality data  
4D Viewer Visualize large-scale 3D/4D image stacks, multi-channel and multi-dimensional, up to Terabyte volumes  
Analysis Pipeline The Analysis Pipeline is an intuitive, fast and flexible tool to tailor the processing of image data to your needs  


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