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Manual segmentation of micronuclei and nuclei in 3D is an extremely tedious task even for just one entire embryo. Automated segmentation and analysis of these types of nuclei opens up the possibility to analyze genotoxic stress and the effect of therapeutic drugs.
This solution can be used to quantify the number and volume of nuclei and micronuclei separately. First, a deep learning model is used to segment the micronuclei and nuclei in entire z-stacks acquired of whole embryos. Then, 3D analysis is done to quantify features such as the number and volume of nuclei and micronuclei. The results are visualized in graphs such as the histograms shown below.
Histogram of nuclei volume.
Histogram of micronuclei volume.