Various applications require a process named stitching and alignment as a prerequisite step for the visualization of 3D or 4D volumetric files. Here, every plane of the resulting image is combined of a number of tiles acquired separately.
ZEISS arivis Pro (formerly Vision4D) is the unique tool for effective stitching and alignment of hundreds to thousands of multiple single files (tiles), which may create volumes of several hundred gigabyte or even terabyte in total file size.
The arivis Tile Sorter allows for a complete interactive adjustment for stitching.
Courtesy of M. Paoli, Galizia Lab, University of Konstanz, Germany, Image acquisition with ZEISS LSM with Airyscan
Image Details: The brain, thoracic and abdominal ganglia of the cockroach are joined together by bilateral connective bundles of ascending and descending interneurons forming the ventral nerve cord. In this preparation, left and right connectives were individually labelled (Alexa 488: green, Alexa 647: magenta) posteriorly to the suboesophageal ganglion to observe the extension of their innervation within the different neurophils, and throughout the ipsi- and contralateral parts of the brain (DNA labelled with DAPI: cyan). Imaging was performed using Tiling and Stitching to capture the complete volume (3×2.3× 0.26 mm). 3D animation of the complete dataset was done with ZEISS arivis Pro (formerly Vision4D), ideal for rendering and analyzing large datasets. The 4D viewer in ZEISS arivis Pro (Vision4D) can be configured to adjust the appearance of individual channels independently to highlight specific features.
The arivis Scientific Image Analysis Platform is a flexible computing universe that scales, parallelizes, and streamlines all imaging workflows. With arivis' easy sharing functions your organization can enjoy data proficiency and efficiency at new levels. arivis includes integrated toolsets that handle everything, from file storage format to project and user-specific computations, to reporting. The platform's hubs connect datasets and manage central imaging databases and raw data exposure, while also enabling Machine Learning and AI routines.
Extract results from your scientific images with ZEISS arivis Pro. Make the most of hardware power adjustments and smooth interactivity on microscopy datasets of virtually unlimited size. The software unites multiple tools for visualization and advanced analysis into one easy-to-learn user environment for high productivity. Extend your possibilities with the Python coding feature and a multitude of supported libraries and APIs. Optional Virtual Reality interface.