TILING & STITCHING

Various applications require a process named stitching and alignment as a prerequisite step for the visualization of 3D or 4D volumetric files. Here, every plane of the resulting image is combined of a number of tiles acquired separately.
arivis Vision4D is the unique tool for effective stitching and alignment of hundreds to thousands of multiple single files (tiles), which may create volumes of several hundred gigabyte or even terabyte in total file size.
The arivis Tile Sorter allows for a complete interactive adjustment for stitching.

• Arrange single and isolated image tiles to a large stitched volume
• Plane shift for tile stacks
• Supports source data with tiles organized as scopes
• Support for manually rotated tiles
• Manual as well as automatic stitching and blending
• Blending between overlapping regions
• Methods for aligning
• Grid: Manually define order & overlap based on equally distributed grid
• Manual: Drag and drop single tiles by hand

Courtesy of M. Paoli, Galizia Lab, University of Konstanz, Germany, Image acquisition with ZEISS LSM with Airyscan
Image Details: The brain, thoracic and abdominal ganglia of the cockroach are joined together by bilateral connective bundles of ascending and descending interneurons forming the ventral nerve cord. In this preparation, left and right connectives were individually labelled (Alexa 488: green, Alexa 647: magenta) posteriorly to the suboesophageal ganglion to observe the extension of their innervation within the different neurophils, and throughout the ipsi- and contralateral parts of the brain (DNA labelled with DAPI: cyan). Imaging was performed using Tiling and Stitching to capture the complete volume (3×2.3× 0.26 mm). 3D animation of the complete dataset was done with arivis Vision4D, ideal for rendering and analyzing large datasets. The 4D viewer in arivis Vision4D can be configured to adjust the appearance of individual channels independently to highlight specific features.