Volume Fusion

Neuronal connectome of a whole adult mouse revealed by vDISCO technology

Image Courtesy: vDisco technology, Ertürk Lab, Institute for Stroke and Dementia Research, Klinikum der Universität München, Munich, Germany

Some samples may be too large to be imaged in a single field of view at the desired resolution. Mosaic tiling can commonly workaround this limitation and the arivis Tile Sorter can conveniently stitch such images into a single large volume. In some cases mosaic tiling may not be sufficient and it may be necessary to move/turn the sample to image the complete volume which can prevent stitching methods that rely on translation alone. The new arivis Volume Fusion Module overcomes these limitations and allows to fuse volumes based on landmarks and/or the free movement, rotation and scaling of a surface representation.

Benefits:

  • Fusing a number of large data sets into one huge data set
  • Easy analysis of the complete sample in one data set
  • Measurement of long structures (neurons and vessels) using InViewR, even in large tissues which are usually too big for a single microscope field of view
  • Merge volumes as separate channels for multimodal imaging

Applications:

  • Create a single large 3D data set from multiple sub volumes
  • Work around light penetration issues in light sheet microscopy by combining different views
  • Combine different imaging modalities or resolutions of the same sample

Use Case:

Image Courtesy: vDisco technology, Ertürk Lab, Institute for Stroke and Dementia Research, Klinikum der Universität München, Munich, Germany