Colocalization Analysis

arivis Vision4D: Colocaliaztion
In fluorescence microscopy, colocalization refers to observation of the spatial overlap between two (or more) different fluorescent labels, each having a separate emission wavelength, to see if the different "targets" are located in the same area of the cell or very near to one another.
The definition can be split into two different phenomena, co-occurrence, which refers to the presence of two (possibly unrelated) fluorophores in the same voxel, and correlation, a much more significant statistical relationship between the fluorophores indicative of a biological interaction.


The Colocalization Palette in arivis Vision4D enables you to analyze the colocalization of voxel intensities in different channels of your image data and provides interactive visualization in the 2D Viewer. Among others, Pearson's correlation coefficient and Manders split coefficients are two of many colocalization coefficients to express the intensity correlation of colocalizing objects in each component of a dual-color image. These coefficients measure the amount or degree of colocalization, or rather correlation and co-occurrence respectively. 


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