Live-cell imaging of meiotic events in C. elegans. With its expertise on correlative light and electron microscopy (CLEM), the laboratory of Prof. Dr. Thomas Müller-Reichert at the TU Dresden is committed to study the details of meiotic cell divisions and the function of the spindle apparatus during this process using the model organism C. elegans. Whereas mitotic spindle dynamics have been studied in detail within C. elegans, the dynamics during male meiosis remain mainly elusive.
After image acquisition, the individual z-stacks had to be stitched to generate a 3D image of the mouse brain. However, the researchers were confronted with the enormous data set of ~100 GB for one channel only. This difficulty was only solved by arivis Vision4D.
Integrating subcellular information into the context of whole tissues is important for understanding cellular networks and their contribution to normal and diseased conditions. Challenged by the size and opacity of tissues, only recent advancement in imaging techniques paved the way to study intact organs at a subcellular resolution.