The Advanced Dynamics Package includes both the moduls to analyse Ratio and FRET.
- Simultaneous evaluation of several channels
- Algorithm for a background correction
- Threshold (optional) for noise reduction
- Calculation and storage of ratio images as gray scale image(s)
- Measurement and visualization of intensity values over time in virtually unlimited user definable ROIs
- Evaluation of changes of medium intensities in ROIs over time in one channel
- Minimum and maximum values of ROIs over time
- Export of data to Excel file formats or as charts to clipboard
- Calculation and exploration of FRET values from three-channel FRET experiments.
- Selection of channels from drop-down list
- Calculation of FRET images per pixel
- Youvan, Gordon and Xia- Algorithmus for FRET and normalized FRET calculation
- XY Offset Alignment for alternation of the original x and y offset values
- Bleed-Through allowing separate entering of bleed-through values for donor and acceptor
- Export of calculated values to *xls or as charts to clipboard