New microscopy technique for isotropic light-sheet imaging published

The research group of Gaudenz Danuser and Reto Fiolka recently published a new light-sheet microscopy technique in the scientific journal Nature Methods. This technique, termed "cleared-tissue axially swept light-sheet microscopy" (ctASLM), enables them to not only achieve subcellular resolution in combination with light-sheet microscopy. It also brings up to tenfold higher resolution in z compared to confocal or other light-sheet microscopy techniques. In their work, they present images that show the best z-resolution ever reported in light-sheet microscopy, without applying super resolution techniques such as SIM, STED or PALM/STORM.


The researchers tried their techniques on various samples and were able to generate images that allow the automatic detection of small-scale objects such as synaptic spines and cell-cell interactions. Kevin Dean, Assistant Professor, Department of Cell Biology, UT Southwestern Medical Center and leading scientist of this work used arivis Vision4D to visualize images acquired with ctASLM. "arivis Vision4D allowed us to dynamically interact with and visualize large datasets (e.g., > 1 TB) without a problem" he reports.


We are proud that our products enable scientists to perform such cutting-edge work and congratulate the whole team for their achievement.


Further Reading:

Chakraborty, T., Driscoll, M.K., Jeffery, E. et al. Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution. Nat Methods 16, 1109–1113 (2019) doi:10.1038/s41592-019-0615-4

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